NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) is a cutting-edge, highly optimized master mix engineered for superior performance in one-step reverse transcription and real-time quantitative PCR (RT-qPCR).
Designed to enable cDNA synthesis and qPCR amplification in a single tube, this master mix ensures streamlined workflows, reduced handling steps, and minimal contamination risk. The formulation of NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) supports the rapid and sensitive detection of RNA targets with unparalleled precision. With a faster cDNA synthesis step of only 1 minute at 60 °C, combined with an accelerated qPCR cycling protocol, this mix significantly reduces assay time while maintaining exceptional sensitivity and reproducibility. Capable of amplifying and detecting up to 7 targets simultaneously in a single reaction, NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) delivers unmatched multiplexing capacity, ideal for high-throughput applications, viral and bacterial pathogen detection, and complex assays.
This master mix is particularly well-suited for demanding molecular biology workflows requiring enhanced performance in the presence of inhibitors commonly found in clinical and environmental samples.
NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) is formulated as a concentrated 2× solution containing all necessary components for robust, high-efficiency multiplex RT-qPCR amplification, including specially engineered enzymes, dNTPs, stabilizers, and PCR enhancers. The mix incorporates a multiple hot-start enzyme activation system, ensuring optimal specificity, sensitivity, and low background signal. Its advanced buffer chemistry and proprietary enzyme engineering further enhance stability and ensure high reproducibility, providing superior results across a wide range of assays. This mix is ROX-free, specifically optimized for probe-based qPCR detection technologies, offering flexibility to adapt to various qPCR instruments.